Recombinant Human MMAB - 50 µg

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mmab50

  • Host : Escherichia coli (E. coli)
  • Clonality : Recombinant protein (non applicable pour clonality)
  • Clone : N/A
  • Isotype : N/A
  • Immunogen : Full-length recombinant human MMAB protein expressed in E. coli
  • Purity : Greater than 80% as determined by SDS-PAGE.
  • Form : Lyophilized from a 0.2 μm filtered solution in 10 mM Hepes, 500 mM NaCl with 5% trehalose, pH 7.4.
  • Molecular weight : Approximately 51 kDa (based on MMAB UniProt data)
  • Uniprot : Q9UBI1
  • Gene id : 4157
  • Background : MMAB (Methylmalonic aciduria type B protein) is involved in adenosylcobalamin (AdoCbl) biosynthesis, essential for mitochondrial methylmalonyl-CoA mutase activity. Mutations lead to methylmalonic aciduria, a metabolic disorder.
  • Field of research : Metabolic disorders, mitochondrial biology, enzymology, genetic diseases
  • Storage conditions : The lyophilized protein is stable at -20°C for up to 1 year. The protein solution can be stored at 2-8°C for up to 1 week under sterile conditions. For extended storage, dilute further in a buffer containing 0.1% BSA (ensure BSA does not interfere with the experiment) and store aliquots at -20°C to -80°C for up to 6 months. Avoid repeated freeze-thaw cycles.
  • Applications : Western Blot, ELISA
  • Dilution : Use appropriate dilution depending on the application and experimental setup (typically 1:500 to 1:2000 for Western Blot; optimization recommended)
  • Target : Human MMAB protein
  • Purification : Affinity chromatography (e.g., Ni-NTA for His-tagged protein) followed by further purification steps to achieve >80% purity
  • Reactivity : Human; cross-reactivity with other species not characterized.

 

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