AffiEXTRACT® Single Nuclei Isolation Kit
This kit is used when your sells are not suitable for scRNA seq because they cannot be harvested intact.
This kit is used for snRNA-seq, single nucleus RNA sequencing, single nuclei RNA sequencing or sNuc-seq, and for profiling gene expression in sigle nuclei from cells which are difficult to isolate.
Advantages of snRNA-seq:
- Compatibility with frozen and archived tissues.
- Preserves gene expression information from rare or difficult-to-isolate cells.
- Enables study of complex, fragile, or multinucleated cells.
Overall, snRNA-seq extraction kit or Single Nuclei Isolation Kit is a robust and versatile method that expands the possibilities for single-cell transcriptomic analysis, especially in scenarios where obtaining viable cells is difficult or impossible. Lieven Gevaert, Bio-ir. Gent
This isolation kit is used as an alternative to single cell RNA seq (scRNA-seq), as it sequences nuclei RNA's instead of intact cells Transcriptomes.
The kit is an advanced tool designed to efficiently isolate and purify nuclei from tissues and cultured cells, overcoming common challenges like aggregation and doublets.:
Nuclei Isolation for Single Nucleus Sequencing (RNA-seq and ATAC-seq)
The kit is essential for preparing high-quality nuclei for single nucleus RNA sequencing and chromatin accessibility assays (ATAC-seq), crucial for understanding gene expression and chromatin dynamics in heterogeneous tissues at a single-cell resolution.
Nuclei Isolation for Epigenetic Studies:
- ChIP-seq (Chromatin Immunoprecipitation Sequencing): The isolated nuclei can be used to study protein-DNA interactions, helping researchers explore epigenetic regulation and modifications such as histone modifications.
- DNA Methylation Analysis: This enables the examination of methylation patterns across different conditions, providing insights into gene regulation, imprinting, and disease mechanisms.
Nuclear Proteomics:
Nuclei Isolation for Mass Spectrometry:
Researchers can utilize isolated nuclei to identify and quantify nuclear proteins, facilitating studies on nuclear functions, including transcription, DNA repair, and replication.
Nuclei Isolation for Drug Screening and Pharmacological Studies:
Nuclear Response to Drugs: The kit can be used to study the effects of various drugs on nuclear functions, including how compounds affect DNA repair, nuclear signaling pathways, or gene transcription, making it valuable in drug development and testing.
Nuclei Isolation for Nuclear Morphology and Structural Studies:
Electron Microscopy: The isolated nuclei are suitable for detailed visualization using electron microscopy, allowing for in-depth analysis of nuclear structure, chromatin organization, and overall nuclear architecture.
Nuclei Isolation for Transcriptomics & Gene Expression:
- qPCR and RT-qPCR: RNA extracted from the isolated nuclei can be used in quantitative PCR studies, enabling precise measurements of gene expression within the nucleus.
- In Situ Hybridization: Enables detection of specific nucleic acid sequences, facilitating the study of gene localization and expression directly within nuclear compartments.
Flow Cytometry (FACS) Analysis:
The kit prepares nuclei for use in flow cytometry, enabling sorting and analysis based on nuclear characteristics, which is vital for cell population studies, including tumor profiling and stem cell research.
Nuclei Isolation for Immunofluorescence Staining:
Researchers can stain the isolated nuclei with fluorescent markers, allowing visualization and study of nuclear proteins and structures, supporting experiments related to protein localization and nuclear compartmentalization.
Nuclei Isolation for Cell Cycle Analysis:
The nuclei isolated with this kit can be used to study the different phases of the cell cycle, providing insights into cell division, growth, and proliferation, which are crucial in cancer research.
Other nuclei isolation kits as of 2025 market overview:
- Nuclei EZ Prep Isolation Kit (Sigma-Aldrich) - Link
- NEBNext® Single Cell/Low Input RNA Library Prep Kit (New England Biolabs) - Link
- Nuclei Isolation Buffer (for Single-Cell RNA Sequencing) - Link
- 10x Genomics Nuclei Isolation Protocols - Link
- Miltenyi Biotec Nuclei Isolation Kit - Link
- Nuclei PURE Prep Kit (Sigma-Aldrich) - Link
- Nuclei EZ Prep Isolation Kit by Sigma-Aldrich:
- Available on Sigma-Aldrich and Krackeler Scientific.
- NEBNext Single Cell/Low Input RNA Library Prep Kit by New England Biolabs:
- You can find more details on NEB's official site or VWR.
- Miltenyi Biotec Nuclei Extraction Solutions:
- Details on nuclei isolation solutions are available at Miltenyi Biotec.
- 10x Genomics Nuclei Isolation Protocols:
- 10x Genomics provides detailed protocols for nuclei isolation compatible with their single-cell platforms. You can explore more on their official website under resources for Single Cell ATAC, RNA Sequencing, and Multiome ATAC + GEX.
The Single Nuclei Isolation Kit from Affigen (AffiExtract) is compatible with following instruments:
- 10x Genomics - High-throughput single-cell sequencing and multi-omics platforms.
- Fluidigm - Microfluidic systems for single-cell genomics and proteomics.
- BD Biosciences - Multi-omics solutions integrating RNA, protein, and CRISPR screening.
- Illumina - Sequencing platforms compatible with single-cell workflows.
- Mission Bio - Single-cell DNA sequencing focusing on genetic heterogeneity.
- Takara Bio - High-throughput RNA sequencing systems.
- Parse Biosciences - Scalable, cost-effective single-cell RNA sequencing.
- Vizgen - Spatial genomics for in situ gene expression mapping.
- Singleron - Comprehensive single-cell sequencing solutions.
- Formulatrix - Automation systems for sample preparation.
- Akoya Biosciences - Spatial biology for tissue imaging and protein expression.
- SeekGene - High-throughput single-cell RNA and DNA sequencing.
- S2 Genomics - Automated tissue dissociation for single-cell prep.
- Canopy Biosciences - Multiplexed imaging for spatial biology.
- Atrandi Biosciences - Microfluidic devices for cell isolation.
- Bio-Rad (Celsee) - Microfluidic-based single-cell isolation and analysis.
- Berkeley Lights - High-throughput cell manipulation and analysis.
- Molecular Devices - Imaging systems for phenotypic screening.
- Menarini Silicon Biosystems - Digital sorting of rare cells.
- Oxford Nanopore Technologies - Long-read sequencing for complex analyses.
- NanoString Technologies - Spatial transcriptomics and proteomics.
- Bionano Genomics - Genome mapping for structural variation analysis.
- Stilla Technologies - Digital PCR for sensitive single-cell detection.
- Single Cell Discoveries - End-to-end single-cell RNA sequencing services.
- Dolomite Bio - Microfluidic systems for scalable single-cell analysis.