Product Components & Selection Guide
Performance
Optimized Hi-Yield T7 In Vitro Transcription Reagents are compatible with templates of varying
lengths, offering superior performance in terms of both yield and purity.
As shown in the figure above, through optimization, the mRNA yield and purity are at the top level
using Hzymes Hi-Yield T7 In Vitro Transcription Reagents compared with several competitors under
6 different length templates. Templates with GGG and AGG as starting sequences both exhibit higher
yields and purity using Hzymes Hi-Yield T7 In Vitro Transcription Reagents.
EL(1000nt~) Product Luc (2000nt~) BK(4000nt~) SP(4000nt~)GGG (5000nt~) SP(4000nt~)AGG
Yield (µg) Purity% Yield (µg) Purity% Yield (µg) Purity% Yield (µg) Purity% Yield (µg) Purity% Yield (µg) Purity%
HZYMES
Performance
HH
Z company
V company
J company
K company
UTP
pUTP
N1-Me-pUTP
UTP
pUTP
N1-Me-pUTP
UTP
pUTP
N1-Me-pUTP
UTP
pUTP
N1-Me-pUTP
UTP
pUTP
N1-Me-pUTP
90.10%
90.00%
89.60%
88.60%
87.30%
86.10%
87.20%
83.70%
82.10%
85.50%
87.60%
88.90%
87.10%
88.00%
87.70%
194.05
186.28
187.1
197.36
188.32
168.87
203.45
191.9
194.21
155.91
145.63
145.42
203.66
198.3
191.97
88.00%
86.70%
86.80%
73.60%
72.30%
68.10%
76.40%
74.90%
75.40%
79.90%
79.20%
77.70%
80.80%
79.50%
81.80%
231.89
210.14
215.62
197.64
176.22
153.61
231.21
212.05
218.05
165.2
131.57
152.22
232.16
211.04
213.75
Product Modified Nucleotides Luc (2000nt~) SP(4000nt~)GGG
Cap AG
Cap 3'OMe
pUTP
N1-Me-pUTP
pUTP
N1-Me-pUTP
78
79
77
80
100%
100%
96.48%
96.16%
179
178
185
182.5
Modified
Nucleotides Cap1 β-Gal
Yield Purity% Capping Rate
Hzymes Hi-Yield T7 In Vitro Transcription Reagents are adapted to different modified nucleotides,
and the products exhibit better yield and purity compared to those of competitors.
Using LC-MS platform for detecting the capping efficiency of the Co-Capping T7 In Vitro Transcription
Reagents, the capping efficiency exceeds 95%.
Yield (µg) Purity% Yield (µg) Purity%
HZYMES
Ordering Information
Products Cat. No. Specification Storage
Temperature
Hi-Yield T7 In Vitro Transcription Reagent
(N1-Me-pUTP)
Hi-Yield T7 In Vitro Transcription Reagent
(pUTP)
Co-Capping T7 In Vitro Transcription Reagent
(pUTP, CAP GAG(3’OMe) )
Co-Capping T7 In Vitro Transcription Reagent
(pUTP, CAP GAG)
Co-Capping T7 In Vitro Transcription Reagent
(N1-Me-pUTP, CAP GAG(3’OMe))
Co-Capping T7 In Vitro Transcription Reagent
(N1-Me-pUTP, CAP GAG)
HBP001505
HBP001506
HBP001507
HBP001508
HBP001509
HBP001510
50T
50T
50T
50T
50T
50T
-25℃ ~ -15℃
-25℃ ~ -15℃
-25℃ ~ -15℃
-25℃ ~ -15℃
-25℃ ~ -15℃
-25℃ ~ -15℃
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