Detecting Depression Biomarkers with MDD CircRNA RT-PCR Detection Kit
Discover how our MDD CircRNA RT-PCR Detection Kit enables precise identification of depression-related circular RNAs like circDYM, circHIPK2, and circSTAG1 using validated primers and protocols based on peer-reviewed research.
The Future of Depression Diagnostics : Using CircRNAs with RT-PCR Detection Kits
Major Depressive Disorder (MDD) affects over 260 million people worldwide , according to the World Health Organization. Despite its prevalence, diagnosing MDD remains largely subjective, relying on clinical interviews and self-reported symptoms. However, emerging research into circular RNAs (circRNAs) has opened new doors for objective, molecular-level diagnostics.
The MDD CircRNA RT-PCR Detection Kit is at the forefront of this revolution. Designed for high specificity and sensitivity, this kit enables researchers and clinicians to detect key circRNA biomarkers associated with depression, including :
- circDYM
- circHIPK2
- circSTAG1
These circRNAs have been identified as critical players in depression pathogenesis through studies published in top-tier journals such as Microbiome , Journal of Extracellular Vesicles , Molecular Psychiatry , and Biological Psychiatry .
circDYM – A Microglia-Regulating Biomarker
According to the 2022 study in the Journal of Extracellular Vesicles , circDYM levels are significantly reduced in both human patients and animal models of chronic unpredictable stress (CUS). This downregulation leads to increased microglial activation via the miR-9/HSP90 pathway, contributing to neuroinflammation and depressive-like behaviors.
Using the MDD CircRNA RT-PCR Detection Kit , researchers can quantify circDYM expression levels and assess its role in immune modulation and potential therapeutic interventions.
circHIPK2 – Gut Microbiome Mediated Regulation
A 2019 Microbiome paper revealed that circHIPK2 plays a central role in the gut-brain axis. Transplantation of microbiota from NLRP3-deficient mice led to improved depressive-like behavior by modulating astrocyte function through circHIPK2 signaling.
This discovery underscores the importance of studying gut-derived circRNAs in psychiatric disorders. Our kit provides tools to explore these interactions further.
circSTAG1 – m6A Methylation and Astrocytic Dysfunction
In Biological Psychiatry (2020) , it was found that circSTAG1 interacts with the m6A methylation pathway , particularly through ALKBH5 and FAAH, affecting astrocytic function. Lower levels of circSTAG1 were correlated with more severe depressive symptoms.
The MDD CircRNA RT-PCR Detection Kit includes optimized primers for detecting circSTAG1, enabling deeper insights into epigenetic regulation in MDD.
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Our detection kit uses divergent primer design to specifically amplify back-spliced junctions—unique to circular RNAs. This ensures high specificity and avoids cross-detection of linear RNA isoforms.
Key Features :
✔️ Validated primers for circDYM , circHIPK2 , circSTAG1.
✔️ Optimized for human and mouse samples.
✔️ Compatible with common qPCR platforms (e.g., ABI StepOne, Roche LightCycler).
✔️ Includes positive controls and RNA extraction protocol.
✔️ High reproducibility and sensitivity.
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From Lab to Clinic – Biomarker Discovery
Researchers can use this kit to identify novel circRNA signatures in large-scale cohort studies. By analyzing blood or post-mortem brain tissue, scientists can correlate circRNA expression profiles with depression severity, treatment response, or relapse risk.
Monitoring Therapeutic Interventions
The kit is ideal for evaluating the efficacy of :
- Psychobiotics (microbiota-based therapies).
- Anti-inflammatory dru gs targeting glial activation.
- Gene therapies involving circRNA delivery via extracellular vesicles.
Personalized Medicine in Psychiatry
With increasing evidence linking specific circRNA dysregulation to individual depressive subtypes, this tool supports precision psychiatry —personalizing treatment strategies based on a patient’s molecular profile.
– Advancing MDD Diagnosis Through Molecular Tools
Depression is not just a psychological condition—it has deep molecular roots. With growing evidence supporting the role of circRNAs in inflammation, neuroplasticity, and the gut-brain axis , tools like the MDD CircRNA RT-PCR Detection Kit are essential for advancing diagnostic precision and therapeutic innovation.
Whether you're conducting preclinical research, clinical trials, or biomarker discovery, our kit offers a reliable, sensitive, and scientifically backed solution.
