Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: human AKT1
AKT2
AKT3
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight:
Uniprot:
Gene id:
Background: Human AKT serine-threonine protein kinase family includes three members AKT1,AKT2, AKT3, which are also often referred to as protein kinase B alpha, beta, and gamma. These highly similar AKT proteins all have an N-terminal pleckstrin homology domain, a serine
threonine-specific kinase domain and a C-terminal regulatory domain. These proteins are phosphorylated by phosphoinositide 3-kinase (PI3K). AKT
PI3K forms a key component of many signalling pathways that involve the binding of membrane-bound ligands such as receptor tyrosine kinases, G-protein coupled receptors, and integrin-linked kinase. These AKT proteins therefore regulate a wide variety of cellular functions including cell proliferation, survival, metabolism, and angiogenesis in both normal and malignant cells. AKT proteins are recruited to the cell membrane by phosphatidylinositol 3,4,5-trisphosphate (PIP3) after phosphorylation of phosphatidylinositol 4,5-bisphosphate (PIP2) by PI3K. Subsequent phosphorylation of both threonine residue 308 and serine residue 473 is required for full activation of the AKT1 protein encoded by this gene.
Field of research:
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC, IF, IP
Dilution: WB: 1:200-1:800; IHC: 1:20-1:100; IF: 1:20-1:100; IP: 1:20-1:100
Target: Pan-AKT
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat