Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: polo-like kinase 2(Drosophila)
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 75-80 kDa
Uniprot: Q9NYY3
Gene id:
Background: Tumor suppressor serine
threonine-protein kinase involved in synaptic plasticity, centriole duplication and G1
S phase transition. Polo-like kinases act by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. Phosphorylates CENPJ, NPM1, RAPGEF2, RASGRF1, SNCA, SIPA1L1 and SYNGAP1. Plays a key role in synaptic plasticity and memory by regulating the Ras and Rap protein signaling: required for overactivity-dependent spine remodeling by phosphorylating the Ras activator RASGRF1 and the Rap inhibitor SIPA1L1 leading to their degradation by the proteasome. Conversely, phosphorylates the Rap activator RAPGEF2 and the Ras inhibitor SYNGAP1, promoting their activity. Also regulates synaptic plasticity independently of kinase activity, via its interaction with NSF that disrupts the interaction between NSF and the GRIA2 subunit of AMPARs, leading to a rapid rundown of AMPAR-mediated current that occludes long term depression. Required for procentriole formation and centriole duplication by phosphorylating CENPJ and NPM1, respectively. Its induction by p53
TP53 suggests that it may participate in the mitotic checkpoint following stress.
Field of research: Epigenetics, Signal Transduction, Metabolism, Developmental biology
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IP
Dilution: WB: 1:500-1:2000; IP: 1:200-1:2000
Target: PLK2
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat