Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: proliferating cell nuclear antigen
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 38 kDa
Uniprot: P12004
Gene id: 5111
Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic(AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response(DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways(PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion(TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Field of research: Epigenetics, Metabolism, Immunology, Cancer, Cell Division and Proliferation
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC, IP
Dilution: WB: 1:500 - 1:2000; IHC: 1:50 - 1:500
Target: PCNA
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat, Rabbit, pig, monkey, zebrafish, hamster