Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: 5'-nucleotidase, ecto(CD73)
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 63 kDa
Uniprot: P21589
Gene id: 4907
Background: NT5E(5'-nucleotidase) is also named as NT5, NTE,CD73,ecto-5'-nucleotidase, and belongs to the 5'-nucleotidase family. It catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP.The enzyme occurs mainly as a dimer and the wide distribution of NT5E in the rat hippocampus, suggesting their involvement in the control of the purinergic signaling(PMID:17619139). This protein has two isoforms with the molecular weight of 63 kDa and 58 kDa and four glycosylation sites with a signalpeptide. It can exsit as a dimer(PMID:17619139). It can be detected the band of 55 kDa,64 kDa, 70 kDa, 110 kDa by western blot(PMID:17619139; 12370277; 21533188). Defects in NT5E are the cause of calcification of joints and arteries(CALJA).
Field of research: Metabolism, Cardiovascular, Immunology, Cancer, Stem Cells
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB,IHC,FC
Dilution: WB: 1:500-1:5000; IHC: 1:50-1:500
Target: NT5E
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat