Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: cyclin-dependent kinase 5
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 33 kDa
Uniprot: Q00535
Gene id: 1020
Background: Proline-directed serine
threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins. Phosphorylates SRC, NOS3, VIM
vimentin, p35
CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM
MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP
NFAT5, MAPT
TAU, MAP1B, histone H1, p53
TP53, HDAC1, APEX1, PTK2
FAK1, huntingtin
HTT, ATM, MAP2, NEFH and NEFM. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Activated by interaction with CDK5R1(p35) and CDK5R2(p39), especially in post-mitotic neurons, and promotes CDK5R1(p35) expression in an autostimulation loop. Phosphorylates many downstream substrates such as Rho and Ras family small GTPases(e.g. PAK1, RAC1, RHOA, CDC42) or microtubule-binding proteins(e.g. MAPT
TAU, MAP2, MAP1B), and modulates actin dynamics to regulate neurite growth and
or spine morphogenesis. Phosphorylates also exocytosis associated proteins such as MCAM
MUC18, SEPT5, SYN1, and CDK16
PCTAIRE1 as well as endocytosis associated proteins such as DNM1, AMPH and SYNJ1 at synaptic terminals. In the mature central nervous system(CNS), regulates neurotransmitter movements by phosphorylating substrates associated with neurotransmitter release and synapse plasticity; synaptic vesicle exocytosis, vesicles fusion with the presynaptic membrane, and endocytosis. Promotes cell survival by activating anti-apoptotic proteins BCL2 and STAT3, and negatively regulating of JNK3
MAPK10 activity. Phosphorylation of p53
TP53 in response to genotoxic and oxidative stresses enhances its stabilization by preventing ubiquitin ligase-mediated proteasomal degradation, and induces transactivation of p53
TP53 target genes, thus regulating apoptosis. Phosphorylation of p35
CDK5R1 enhances its stabilization by preventing calpain-mediated proteolysis producing p25
CDK5R1 and avoiding ubiquitin ligase-mediated proteasomal degradation. During aberrant cell-cycle activity and DNA damage, p25
CDK5 activity elicits cell-cycle activity and double-strand DNA breaks that precedes neuronal death by deregulating HDAC1. DNA damage triggered phosphorylation of huntingtin
HTT in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Phosphorylation of PXN reduces its interaction with PTK2
FAK1 in matrix-cell focal adhesions(MCFA) during oligodendrocytes(OLs) differentiation. Negative regulator of Wnt
beta-catenin signaling pathway. Activator of the GAIT(IFN-gamma-activated inhibitor of translation) pathway, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells; phosphorylates the linker domain of glutamyl-prolyl tRNA synthetase(EPRS) in a IFN-gamma-dependent manner, the initial event in assembly of the GAIT complex. Phosphorylation of SH3GLB1 is required for autophagy induction in starved neurons. Phosphorylation of TONEBP
NFAT5 in response to osmotic stress mediates its rapid nuclear localization. MEF2 is inactivated by phosphorylation in nucleus in response to neurotoxin, thus leading to neuronal apoptosis. APEX1 AP-endodeoxyribonuclease is repressed by phosphorylation, resulting in accumulation of DNA damage and contributing to neuronal death. NOS3 phosphorylation down regulates NOS3-derived nitrite(NO) levels. SRC phosphorylation mediates its ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. May regulate endothelial cell migration and angiogenesis via the modulation of lamellipodia formation. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. The complex p35
CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL
BMAL1 heterodimer in association with altered stability and subcellular distribution.
Field of research: Neuroscience, Cell Division and Proliferation, Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB
Dilution: WB: 1:500-1:2000
Target: CDK5
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat