Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: amphiregulin
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 43 kDa
Uniprot: P15514
Gene id: 374
Background: Amphiregulin(AREG) is one of the ligands of the epidermal growth factor receptor(EGFR). AREG plays a central role in mammary gland development and branching morphogenesis in organs and is expressed both in physiological and in cancerous tissues. The AREG protein is synthesized as a 252-amino acid transmembrane precursor, pro-AREG. At the plasma membrane, pro-AREG is subjected to sequential proteolytic cleavages within its ectodomain and is then released as the soluble AREG protein. Depending on the cell type and microenvironment, AREG can be produced in multiple cellular and mature forms using alternative pro-AREG cleavage sites and glycosylation motifs. Post-translastional modfications of 50-kDa pro-AREG produces a major soluble 43-kDa form, 28-, 26-, 16-kDa membrane anchored forms, and soluble 21-, 19-, and 9-kDa forms(PMID: 9642297).
Field of research: Immunology, Cardiovascular, Signal Transduction
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, IF, WB, IHC,IP
Dilution: WB: 1:200-1:2000; IHC: 1:50-1:500; IP: 1:1000-1:10000; IF: 1:10-1:100
Target: AREG
Purification: Immunogen affinity purified
Reactivity: Human