pHTS-hygro-NFAT is a versatile reporter plasmid designed for the study of NFAT (Nuclear Factor of Activated T-cells) signaling pathway activity. This plasmid combines the NFAT-responsive promoter with a hygromycin resistance gene, allowing for efficient selection and maintenance of stable cell lines. The pHTS-hygro-NFAT construct enables high-throughput screening (HTS) of NFAT pathway modulators, making it ideal for drug discovery and therapeutic research targeting immune response regulation. The inclusion of a hygromycin resistance marker ensures stable and long-term expression of the reporter in mammalian cells, enabling accurate and reproducible results. Use pHTS-hygro-NFAT to investigate NFAT-mediated gene expression in various cell types, including T-cells, for applications in immunology, oncology, and autoimmune disease research. - kit