pHTS-hygro-GAS is a high-performance cis element luciferase reporter plasmid specifically engineered to monitor the transcriptional activity of the GAS (Gamma-Activated Sequence) element, a key regulatory site responsive to interferon-gamma (IFN-γ) signaling. This vector contains multiple GAS response elements upstream of a minimal promoter driving the expression of the luciferase reporter gene, allowing precise, real-time quantification of STAT1-mediated transcriptional activation. Equipped with a hygromycin resistance gene, pHTS-hygro-GAS is ideal for generating stable cell lines and conducting high-throughput screening (HTS) in immune signaling and cytokine response studies. Reliable, sensitive, and easy to transfect, this reporter plasmid is a powerful tool for analyzing JAK/STAT pathway activity and studying inflammation, immunity, and cancer-related gene regulation. - kit