
What is 8-OHdG?
8-hydroxy-2’-deoxyguanosine (8-OHdG) is a biomarker that indicates DNA damage caused by reactive oxygen species (ROS). When oxidative stress damages DNA, 8-OHdG is one of the most common products, making it an essential marker for assessing cellular damage. Measuring its levels can help understand the effects of oxidative stress in many conditions, including:
- Cancer : Damage to DNA may lead to mutations and tumor formation.
- Neurodegenerative diseases : Oxidative damage is linked to diseases like Alzheimer’s and Parkinson’s.
- Aging : Increased oxidative stress can accelerate cellular aging.
How Does the 8-OHdG ELISA Kit Work?
The 8-OHdG ELISA Kit uses a competitive enzyme-linked immunosorbent assay to measure 8-OHdG in samples such as urine, plasma, serum, or cell lysates. Here’s how the process works step by step:
- Sample Preparation :
Biological samples are diluted as needed to match the detection range of the kit.
- Competitive Binding :
The microplate is pre-coated with anti-8-OHdG antibodies.
A fixed amount of enzyme-conjugated 8-OHdG competes with 8-OHdG in the sample for antibody binding.
- Washing :
After incubation, unbound materials are washed away, ensuring only the antibody-bound complex remains.
- Signal Detection :
A substrate is added, which reacts with the enzyme to produce a color change. The intensity of the color is inversely proportional to the amount of 8-OHdG in the sample.
- Quantification :
The absorbance is measured using a spectrophotometer, and the concentration of 8-OHdG is calculated based on a standard curve.

High Sensitivity :
Detects very low levels of 8-OHdG, down to 0.5 ng/mL, making it suitable for research where precision is critical.
Wide Application :
Can analyze oxidative stress in samples from humans, animals, and cell culture experiments.
Reproducibility :
Provides consistent results across multiple assays.
Advantages of the 8-OHdG ELISA Kit
Specificity:
The use of highly specific monoclonal antibodies minimizes cross-reactivity with other molecules.
Ease of Use:
Designed for researchers at any level of expertise, the kit simplifies the process of detecting 8-OHdG.
Cost-Effective:
Provides accurate results without the need for expensive instruments like mass spectrometry.
Wide Dynamic Range:
The kit can measure both low and high concentrations of 8-OHdG in various sample types.
How the 8-OHdG ELISA Kit Helps Researchers
The 8-OHdG ELISA Kit simplifies the measurement of oxidative DNA damage, enabling scientists to focus on their research questions. Whether you're investigating disease mechanisms, testing new drugs, or studying environmental impacts, this kit provides the reliability and precision needed to generate meaningful results.

Anya Petrova-Volkova, Ph.D.,
Is a trailblazer in the field of oxidative stress. Driven by a deep curiosity about the aging process, she has dedicated her career to unraveling the role of 8-OHdG in neurodegenerative diseases. Her groundbreaking research has culminated in the development of a cutting-edge ELISA kit, revolutionizing the study of oxidative DNA damage.