Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: IL2-inducible T-cell kinase
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight:
Uniprot:
Gene id:
Background: Tyrosine kinase that plays an essential role in regulation of the adaptive immune response. Regulates the development, function and differentiation of conventional T-cells and nonconventional NKT-cells. When antigen presenting cells(APC) activate T-cell receptor(TCR), a series of phosphorylation lead to the recruitment of ITK to the cell membrane, in the vicinity of the stimulated TCR receptor, where it is phosphorylated by LCK. Phosphorylation leads to ITK autophosphorylation and full activation. Once activated, phosphorylates PLCG1, leading to the activation of this lipase and subsequent cleavage of its substrates. In turn, the endoplasmic reticulum releases calcium in the cytoplasm and the nuclear activator of activated T-cells(NFAT) translocates into the nucleus to perform its transcriptional duty. Phosphorylates 2 essential adapter proteins: the linker for activation of T-cells
LAT protein and LCP2. Then, a large number of signaling molecules such as VAV1 are recruited and ultimately lead to lymphokine production, T-cell proliferation and differentiation.
Field of research:
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, IHC, WB
Dilution: WB: 1:200-1:2000; IHC: 1:20-1:200
Target: ITK
Purification: Immunogen affinity purified
Reactivity: Human