Host : Mouse
Clonality: Monoclonal
Clone: 5H5
Isotype: IgG2b
Immunogen: vascular endothelial growth factor A
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 45 kDa
Uniprot: P15692
Gene id:
Background: VEGFA, also named as VEGF or VPF, belongs to the PDGF
VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1
VEGFR1 and KDR
VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are a cause of susceptibility to microvascular complications of diabetes type 1(MVCD1). VEGFA has 13 isoforms with MW 16kd to 45kd. Some isoforms has homodimer form, such as VEGFA189(38kd), VEFGA110(34kd). VEGFA is a 34kd to 42kd, dimeric, disulfide-bound glycoprotein. VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids(PMID:15602010). This antibody can recognize all the isoforms of VEFGA.
Field of research: Signal Transduction, Cardiovascular, Cancer, Immunology, Developmental biology
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC
Dilution: WB: 1:1000-1:4000; IHC: 1:50-1:500
Target: VEGFA
Purification: protein A+G purified
Reactivity: Human