Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: vaccinia related kinase 2
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 67 kDa
Uniprot: Q86Y07
Gene id: 7444
Background: Serine
threonine kinase that regulates several signal transduction pathways. Isoform 1 modulates the stress response to hypoxia and cytokines, such as interleukin-1 beta(IL1B) and this is dependent on its interaction with MAPK8IP1, which assembles mitogen-activated protein kinase(MAPK) complexes. Inhibition of signal transmission mediated by the assembly of MAPK8IP1-MAPK complexes reduces JNK phosphorylation and JUN-dependent transcription. Phosphorylates 'Thr-18' of p53
TP53, histone H3, and may also phosphorylate MAPK8IP1. Phosphorylates BANF1 and disrupts its ability to bind DNA and reduces its binding to LEM domain-containing proteins. Downregulates the transactivation of transcription induced by ERBB2, HRAS, BRAF, and MEK1. Blocks the phosphorylation of ERK in response to ERBB2 and HRAS. Can also phosphorylate the following substrates that are commonly used to establish in vitro kinase activity: casein, MBP and histone H2B, but it is not sure that this is physiologically relevant. Isoform 2 phosphorylates 'Thr-18' of p53
TP53, as well as histone H3. Reduces p53
TP53 ubiquitination by MDM2, promotes p53
TP53 acetylation by EP300 and thereby increases p53
TP53 stability and activity.
Field of research: Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IP
Dilution: WB: 1:500-1:5000;IP: 1:200-1:2000
Target: VRK2
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat