Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: ubiquitin-conjugating enzyme E2T(putative)
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 24 kDa
Uniprot: Q9NPD8
Gene id: 28089
Background: The ubiquitin(Ub)-mediated protein degradation pathway involves three sequential enzymatic steps that facilitate the conjugation of Ub to specific protein substrates. The first step requires ATP-dependent activation of the C-terminus of Ub and the assembly of multi-Ubs by Ub-activating enzyme E1. The ubiquitin-conjugating enzyme E2, catalytic(UBCc) domain, is then conjugated to Ubs, through a thiol-ester linkage between a conserved cysteine and the C-terminus of Ub, to generate an intermediate Ub-E2 complex. Then the E3, a ligase, catalyzes the transfer of Ub from E2 to the appropriate substrate. This pathway regulates many fundamental cellular processes. There are also other E2s which form thiol-ester linkages without the use of E3s as well as several UBC homologs(TSG101, Mms2, Croc-1 and similar proteins), which lack the active site cysteine essential for ubiquitination and appear to function in DNA repair pathways.
Field of research: Epigenetics, Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IP
Dilution: WB: 1:200-1:2000; IP: 1:200-1:2000
Target: UBE2T
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat