Host : Mouse
Clonality: Monoclonal
Clone: 2A9
Isotype: IgG2b
Immunogen: tumor necrosis factor(TNF superfamily, member 2)
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 22 kDa
Uniprot: P01375
Gene id: 7124
Background: TNF, as also known as TNF-alpha, or cachectin, is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor(TNF) superfamily. It is expressed as a 26 kDa membrane bound protein and is then cleaved by TNF-alpha converting enzyme(TACE) to release the soluble 17 kDa monomer, which forms homotrimers in circulation. It is produced chiefly by activated macrophages, although it can be produced by many other cell types such as CD4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils, and neurons. It can bind to, and thus functions through its receptors TNFRSF1A
TNFR1 and TNFRSF1B
TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.
Field of research: Signal Transduction, Cardiovascular, Cancer, Immunology, Neuroscience
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, WB, IHC
Dilution: WB: 1:500-1:5000; IHC: 1:200-1:1000
Target: TNF alpha
Purification: Protein A+G purification
Reactivity: Human, Mouse, Rat