Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: THO complex 2
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 150 kDa
Uniprot: Q8NI27
Gene id: 57187
Background: Required for efficient export of polyadenylated RNA and spliced mRNA. Acts as component of the THO subcomplex of the TREX complex which is thought to couple mRNA transcription, processing and nuclear export, and which specifically associates with spliced mRNA and not with unspliced pre-mRNA. TREX is recruited to spliced mRNAs by a transcription-independent mechanism, binds to mRNA upstream of the exon-junction complex(EJC) and is recruited in a splicing-and cap-dependent manner to a region near the 5' end of the mRNA where it functions in mRNA export to the cytoplasm via the TAP
NFX1 pathway. The TREX complex is essential for the export of Kaposi's sarcoma-associated herpesvirus(KSHV) intronless mRNAs and infectious virus production. THOC2(and probably the THO complex) is involved in releasing mRNA from nuclear speckle domains. Required for NXF1 localization to the nuclear rim. Plays a role for proper neuronal development.
Field of research: Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, IP, WB, IF
Dilution: WB: 1:500-1:2000; IP: 1:200-1:1000; IF: 1:20-1:200
Target: THOC2
Purification: Immunogen affinity purified
Reactivity: Human