Host : Mouse
Clonality: Monoclonal
Clone: 9E6
Isotype: IgG1
Immunogen: phosphatase and tensin homolog
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 47 kDa
Uniprot: P60484
Gene id: 5728
Background: This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3,4,5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT
PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.
Field of research: Signal Transduction, Metabolism, Cardiovascular, Immunology, Cancer, Neuroscience, Stem Cells, Cell Division and Proliferation
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, IHC, WB, IF
Dilution: WB: 1:1000-1:4000; IHC: 1:200-1:1000; IF: 1:20-1:200
Target: PTEN
Purification: Protein A+G purification
Reactivity: Human, Mouse