Host : Rabbit
Clonality: Polyclonal
Clone:
Isotype: IgG
Immunogen: actin, beta
Purity: ≥95% as determined by SDS-PAGE
Form: Liquid
Molecular weight: 42 kDa
Uniprot: P60709
Gene id: 60
Background: Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha(ACTC1, cardiac muscle 1), alpha 1(ACTA1, skeletal muscle) and 2(ACTA2, aortic smooth muscle), beta(ACTB), gamma 1(ACTG1) and 2(ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2(rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins.
Field of research: Cell Division and Proliferation, Cardiovascular, Metabolism
Storage conditions: PBS with 0.02% sodium azide and 50% glycerol pH 7.3, -20°C for 12 months(Avoid repeated freeze
thaw cycles.)
Applications: ELISA, IF, WB, IHC, IP
Dilution: WB: 1:500-1:5000; IHC: 1:20-1:500; IF: 1:50-1:500;IP: 1:500-1:2000
Target: beta actin
Purification: Immunogen affinity purified
Reactivity: Human, Mouse, Rat