Camel Serum Amyloid A (SAA) ELISA Assay – 96-Well Plate

The Camel Serum Amyloid A (SAA) ELISA Kit is a quantitative sandwich enzyme-linked immunosorbent assay designed for the detection and quantification of SAA concentrations in camel serum, plasma, or other biological fluids. This kit is optimized for high specificity and sensitivity in Camelidae species, enabling the accurate assessment of acute-phase response and systemic inflammation markers.

Key Features:

• Format: 96-well microplate, precoated with a monoclonal anti-Camel SAA capture antibody.
• Detection: HRP-conjugated polyclonal antibody specific to camel SAA.
• Standard Range: Typically 0.1 – 100 ng/mL (exact range may vary by lot; refer to the datasheet).
• Sensitivity: <10 pg/mL.
• Assay Time: 3.5–4 hours total (2-hour sample incubation, 1-hour detection antibody incubation, 30-min substrate reaction).
• Sample Volume: 50–100 µL per well.
• Detection Method: Colorimetric measurement at 450 nm with wavelength correction at 570–630 nm (reference).

Assay Principle:

 This ELISA employs a solid-phase sandwich format. Camel SAA in the sample binds to immobilized monoclonal antibodies specific for SAA on the 96-well plate. Following a wash step, HRP-conjugated detection antibodies bind to the captured antigen. After further washing, TMB substrate is added, producing a colorimetric signal proportional to the SAA concentration. The reaction is stopped with sulfuric acid, and absorbance is measured at 450 nm. A standard curve is constructed from recombinant camel SAA standards to interpolate unknown sample concentrations.

Validation:

• Cross-reactivity and interference tested across multiple camel sera from healthy and diseased individuals.
• Intra- and inter-assay CV <10%.
• Recovery rate: 90–110%.
• Linearity confirmed across serial dilutions of high-concentration camel serum samples.

Applications:

• Monitoring of acute phase response in Camelus dromedarius and Camelus bactrianus.
• Research on infectious diseases, inflammatory conditions, and stress physiology in camels.
• Biomarker studies in veterinary and zoological diagnostics.

Storage and Stability:

• Store the unopened kit at 2–8°C.
• Once opened, components should be used within the validity period and stored as instructed in the manual.
• Avoid repeated freeze-thaw cycles of standards and samples.

Kit Components:

• Pre-coated 96-well ELISA plate
• Camel SAA Standard (lyophilized)
• Sample Diluent, Assay Buffer
• HRP-conjugated Detection Antibody
• Wash Buffer Concentrate (20x)
• TMB Substrate Solution
• Stop Solution (0.5 M H₂SO₄)
• Plate Sealers and Protocol Manual