NZCYM Broth in Microbiology Culture Media

NZCYM broth, also known as New Zealand casein yeast magnesium sulfate broth, is a rich and complex medium used in microbiology for the cultivation of various microorganisms. This medium is particularly beneficial for the growth of Escherichia coli and lambda bacteriophage​. The composition of NZCYM broth includes Casamino acids, Tryptone, Yeast extract, NaCl, and MgSO4 anhydrous, with a final pH of 7.0 ± 0.2 at 25°C​. To prepare NZCYM broth, 22 grams of the powdered medium are typically suspended in 1 liter of distilled water1. It is recommended to store the medium dry at room temperature1. NZCYM broth finds applications in various fields such as microbiology, molecular biology, and industry for tasks like the preparation and recovery of competent cells and culture media for molecular biology purposes​. This medium is known for its ability to support microbial growth effectively, making it a superior choice for increasing yields of phage Lambda and cultivating E. coli and lambda bacteriophag. For those interested in acquiring NZCYM broth, it is available in different quantities ranging from 250g to 6 x 1kg with corresponding prices. Additionally, Formedium Ltd offers custom-made media production services tailored to specific end-user specification. International shipping is available, but customers should be aware that import taxes are their responsibility along with any applicable customs restrictions that may apply​. NZCYM broth stands out as a versatile and effective medium in microbiology culture media due to its rich composition and suitability for various microorganism cultures.

Representative NZCYM agar plate showing the three categories of phage spots: clear (+++), turbid (++), and no reaction (—), on the lawn of host E. coli O157:H7