Product Components & Selection Guide

Performance

Optimized Hi-Yield T7 In Vitro Transcription Reagents are compatible with templates of varying

lengths, offering superior performance in terms of both yield and purity.

As shown in the figure above, through optimization, the mRNA yield and purity are at the top level 

using Hzymes Hi-Yield T7 In Vitro Transcription Reagents compared with several competitors under 

6 different length templates. Templates with GGG and AGG as starting sequences both exhibit higher 

yields and purity using Hzymes Hi-Yield T7 In Vitro Transcription Reagents.

EL(1000nt~) Product Luc (2000nt~) BK(4000nt~) SP(4000nt~)GGG (5000nt~) SP(4000nt~)AGG

Yield (µg) Purity% Yield (µg) Purity% Yield (µg) Purity% Yield (µg) Purity% Yield (µg) Purity% Yield (µg) Purity%

HZYMES

Performance

HH

Z company

V company

J company

K company

UTP

pUTP

N1-Me-pUTP

UTP

pUTP

N1-Me-pUTP

UTP

pUTP

N1-Me-pUTP

UTP

pUTP

N1-Me-pUTP

UTP

pUTP

N1-Me-pUTP

90.10%

90.00%

89.60%

88.60%

87.30%

86.10%

87.20%

83.70%

82.10%

85.50%

87.60%

88.90%

87.10%

88.00%

87.70%

194.05

186.28

187.1

197.36

188.32

168.87

203.45

191.9

194.21

155.91

145.63

145.42

203.66

198.3

191.97

88.00%

86.70%

86.80%

73.60%

72.30%

68.10%

76.40%

74.90%

75.40%

79.90%

79.20%

77.70%

80.80%

79.50%

81.80%

231.89

210.14

215.62

197.64

176.22

153.61

231.21

212.05

218.05

165.2

131.57

152.22

232.16

211.04

213.75

Product Modified Nucleotides Luc (2000nt~) SP(4000nt~)GGG

Cap AG

Cap 3'OMe

pUTP

N1-Me-pUTP

pUTP

N1-Me-pUTP

78

79

77

80

100%

100%

96.48%

96.16%

179

178

185

182.5

Modified

Nucleotides Cap1 β-Gal

Yield Purity% Capping Rate

Hzymes Hi-Yield T7 In Vitro Transcription Reagents are adapted to different modified nucleotides, 

and the products exhibit better yield and purity compared to those of competitors.

Using LC-MS platform for detecting the capping efficiency of the Co-Capping T7 In Vitro Transcription

Reagents, the capping efficiency exceeds 95%.

Yield (µg) Purity% Yield (µg) Purity%

HZYMES

Ordering Information

Products Cat. No. Specification Storage

Temperature

Hi-Yield T7 In Vitro Transcription Reagent

(N1-Me-pUTP)

Hi-Yield T7 In Vitro Transcription Reagent

(pUTP)

Co-Capping T7 In Vitro Transcription Reagent

(pUTP, CAP GAG(3’OMe) )

Co-Capping T7 In Vitro Transcription Reagent

(pUTP, CAP GAG)

Co-Capping T7 In Vitro Transcription Reagent

(N1-Me-pUTP, CAP GAG(3’OMe))

Co-Capping T7 In Vitro Transcription Reagent

(N1-Me-pUTP, CAP GAG)

HBP001505

HBP001506

HBP001507

HBP001508

HBP001509

HBP001510

50T

50T

50T

50T

50T

50T

-25℃ ~ -15℃

-25℃ ~ -15℃

-25℃ ~ -15℃

-25℃ ~ -15℃

-25℃ ~ -15℃

-25℃ ~ -15℃

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Production Center: Precision Medical Industry Base, Wuhan, China

+86 021-60879177 | info@hzymes.com

www.hzymesbiotech.com

HZYMES BIOTECHNOLOGY CO.,LTD