PNGase F (100U/μL)

PNGase F (100 U/μL) is a recombinant glycosidase that cleaves between the innermost N-acetylglucosamine (GlcNAc) and the asparagine residue of N-linked glycans. Supplied at 100 units per microliter, it delivers high activity for analytical, structural, and biopharmaceutical applications.

• Concentrated stock (100 U/μL) for precise dosing and cost-effective workflows.
• High specificity – removes high-mannose, hybrid, and complex N-glycans.
• Compatible with denatured or native proteins, depending on accessibility of glycosylation sites.
• Recombinant, animal-free production ensures batch-to-batch consistency.
• Active under mild conditions (pH 7.5–8.6, 37 °C).

Specifications

• Enzyme class : EC 3.5.1.52 (Peptide-N-glycosidase F)
• Concentration : 100 U/μL (Units defined by release of glycans from glycoprotein substrate)
• Molecular weight : ~34 kDa
• Formulation : Supplied in stabilizing buffer (glycerol-free variants available)
• Storage : −20 °C; avoid repeated freeze–thaw cycles