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µPREP™ Half Fraser Broth ISO (+FAC)

Product Code: MPB004

A sterile, ready to reconstitute primary enrichment medium for the isolation of Listeria spp. from food samples.

µPrep™ Half Fraser Broth ISO (+FAC) is a sterile, ready to reconstitute primary enrichment medium for the isolation of Listeria spp. from foodstuffs formulated according to ISO 11290.

Listeria spp. are able to hydrolyse the aesculin to form aesculetin, which reacts with the FAC resulting in a black precipitate and a visible positive reaction. However, Enterococci can also perform this reaction, so further plating is required onto an isolation medium such as Harlequin™ Listeria Chromogenic Agar ISO (HAL010) or Pinnacle™ Listeria Chromogenic Agar ISO (PIN001). Selectivity is provided by lithium chloride, acriflavine and nalidixic acid.

µPREP™ Half Fraser Broth ISO (+FAC) is supplied as a ready-to-reconstitute bag, sufficient for the preparation of 20 litres of Half Fraser Broth ISO (+FAC).

µPREP™ bags are connected to a reverse osmosis (RO)/deionised water supply and the water is sterilised as it is pumped via a membrane filter into the bag.

Related Sector(s)

Related Organism(s)

  • Listeria