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Perfringens Agar (TSC)

Product Code: LAB194

Tryptose Sulphite Cycloserine Agar

Application(s): Industrial, Food
Organism(s): Clostridia

Perfringens Agar Base is a nutrient medium to which egg yolk emulsion (X073) and cycloserine (X194) are added for the preparation of Tryptose Sulphite Cycloserine (TSC) Agar.

Sodium metabisulphite and ferric ammonium citrate are used as an indicator of sulphite reduction by Clostridium perfringens. The reduction of sulphite by C.perfringens produces black colonies and the egg yolk emulsion incorporated into the media detects the lecithinase activity of this bacteria. However not all strains produce lecithinase and therefore black lecithinase positive and black lecithinase negative colonies should be considered as presumptive C.perfringens.

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