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CSEB - Cronobacter sakazakii Enrichment Broth

Product Code: LAB081

A broth for the isolation of Cronobacter sakazakii from milk and milk products as described in ISO/TS 22964:2006(E).


Cronobacter sakazakii (formerly Enterobacter sakazakii) is a member of the Enterobacteriaceae family and has been associated with serious outbreak infections in neonates (premature infants) which have been fed on infant formula milk. Although rarely causing infections in immunocompetent adults, C. sakazakii has been implicated in sepsis, meningitis and necrotising enterocolitis with a high death rate in neonates. This opportunistic pathogen is common in the environment and its ability to survive desiccation presents a significant risk for post pasteurisation contamination and survival in spray dried milk products.

Based on lauryl sulphate tryptose broth, Cronobacter Sakazakii Enrichment Broth (CSEB) has added sodium chloride for extra selectivity against competing organisms. The antibiotic vancomycin is also added to inhibit Gram-positive organisms such as Staphylococccus aureus which may be able to grow in this medium.

This media formulation is the secondary enrichment broth as currently recommended in the isolation protocol under ISO/TS 22964:2006(E) for the isolation of Enterobacter sakazakii from milk and milk products.

Related Sector(s)

Related Organism(s)

  • Cronobacter sakazakii