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Staphylococcus aureus isolation with Lab M Baird-Parker medium

Lab M’s Baird-Parker Medium (ISO) is designed for the isolation of coagulase-positive staphylococci, including Staphylococcus aureus, from food and animal feedstuffs. The medium is one of the company’s range of microbiological culture media developed to meet specific ISO requirements in the food industry.

Lab M’s Baird-Parker Medium (ISO) is designed for the isolation of coagulase-positive staphylococci, including Staphylococcus aureus, from food and animal feedstuffs. The medium is one of the company’s range of microbiological culture media developed to meet specific ISO requirements in the food industry. As with Lab M’s traditional Baird-Parker medium, the ISO formulation can be used with either Egg Yolk Tellurite or Rabbit Plasma Fibrinogen (RPF). When used with RPF typical S. aureus indicate coagulase activity, appearing on the medium as black colonies surrounded by a zone of precipitation. RPF overcomes any issues with atypical colony forms and requires no further confirmatory tests.When Lab M’s Egg Yolk Tellurite is used with the Baird Parker ISO formulation, presumptive S. aureus demonstrate lecithinase activity, appearing as black colonies surrounded by an opaque zone, encircled by a zone of clearing that is the result of lipase activity. Coagulase (RPF media) or latex agglutination tests are used for confirmation.Baird-Parker medium (ISO) is formulated according to ISO 6888-1:1999. It is in compliance with ISO 6888-2:1999 and 6888-3: 2003 and is tested in accordance with ISO/TS 11133-2:2003(E).

Article first published : 8/1/2009