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Counting Enterobacteriaceae with Lab M

Part of a comprehensive range of culture media produced for the detection and enumeration of Enterobacteriaceae, Lab M’s Violet Red Bile Glucose Agar (VRBGA) delivers presumptive Enterobacteriaceae counts after 18-24 hours incubation.

This medium is used widely in the food and dairy industries as part of critical hygiene monitoring procedures.

Since glucose is fermented by all members of the Enterobacteriaceae, VRBGA enables the growth of all the key organisms of interest including E. coli, Salmonella and Shigella species. The inclusion of bile salts and crystal violet in the medium inhibits Gram positive and non-enteric organisms, while an overlay procedure ensures anaerobic conditions, suppressing the growth of non-fermentative Gram negative bacteria.

Following incubation, the number of red/purple colonies is counted, allowing calculation of the number of Enterobacteriaceae in the original sample.

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Article first published : 24/7/2012